Genetic improvement of the nematicidal fungus Lecanicillium attenuatum against Heterodera glycines by expression of the Beauveria bassiana Cdep1 protease gene

被引：12

作者：

Xie, Ming ^{[1
]}

Zhang, Yan-Jun ^{[1
]}

Zhang, Xiao-Lin ^{[1
]}

Peng, De-Liang ^{[1
]}

Yu, Wen-Bin ^{[1
]}

Li, Qian ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 2016年 / 138卷

基金：

中国国家自然科学基金;

关键词：

Nematophagous fungi; Genetic engineering; Protease; Soybean cyst nematode; EGGS;

D O I：

10.1016/j.jip.2016.06.008

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Lecanicillium attenuatum is an important nematophagous fungus with potential as a biopesticide against plant-parasitic nematodes. The Pr1A-like cuticle-degrading protease (Cdep1) gene originating from the entomopathogenic fungus Beauveria bassiana was transformed into the nematophagous fungus L. attenuatum using a polyethylene-glycol mediated protoplast-based transformation system. Protease activity was increased 0.64- to 1.63-fold 2-10 d after growth in the transformed L. attenuatum. Inhibition of egg-hatching and J2 motility of soybean cyst nematodes (Heterodera glycines) by cell-free fungal culture filtrates were enhanced by 17-76% 2-14 d and 43-152% 1-13 d after incubation, respectively. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：86 / 88

页数：3