Analysis on Enterococcus spp. Resistance and Detection of Resistance Gene vanM in Enterococcus faecium

This study presents an investigation about the distribution of antibiotic resistance in Enterococcus spp. and carried status of the resistance gene vanM in Enterococcus faecalis. Total 506 clinical isolates were detected the drug susceptibility by Disk diffusion test, Read and analysis the result by NCCLS/CLSI 2005. Glycopeptide drug resistance gene vanM was detected by PCR for the resistance strains. Of 506 isolates were urine (33.0%), stool (22.5%), sputum (15.4%), bile (11.3%), pus (10.7%) and others (7.1%) respectively. Enterococcus had a high antibiotic resistance to most antibiotics (p<0.05). The resistance of Enterococcus faecium was higher than Enterococcus faecalis with beta-lactams, quinolones and glycopeptide antibiotics. The carry rate of vanM fragment was 100% and 90% in vancomycin and teicoplanin resistant isolates respectively. It indicates that Enterococcus faecium can cause all kinds of clinical infection and shows a multiple drug resistance trend, vanM gene may play an important role in the resistance mechanisms to glycopeptide antibiotics.