Use of the polymerase chain reaction for Salmonella detection

被引：107
|
作者：
Kwang, J
Littledike, ET
Keen, JE
机构：
[1] U. States Department of Agriculture, Agricultural Research Service, Roman L. Hruska US Meat Anim. R., Clay Center, NE
[2] US Meat Animal Research Center, Clay Center, NE 68933
来源：
LETTERS IN APPLIED MICROBIOLOGY | 1996年 / 22卷 / 01期
关键词：
D O I：
10.1111/j.1472-765X.1996.tb01106.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A primer set of oligonucleotides (S18 and S19) from the ompC gene of Salmonella has been evaluated for specific detection of Salmonella by polymerase chain reaction (PCR). This primer set successfully amplified 40 Salmonella serovars (60 isolates), but not 24 non-Salmonella bacteria (42 isolates) that have been tested so far. The uniqueness of these primer sequences was also confirmed. The sensitivity of PCR detection in extracted chromosomal DNA for Salm. typhimurium was 1 pg. The sensitivity for boiled whole bacteria was 400 cells. The detection of Salm. typhimurium in ground beef samples required 4-6 h enrichment with an initial inocula of 100 bacteria.
引用
收藏
页码：46 / 51
页数：6
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