Three-dimensional differential interference contrast microscopy using synthetic aperture imaging

被引：23

作者：

Kim, Moonseok ^{[1
]}

Choi, Youngwoon ^{[1
]}

Fang-Yen, Christopher ^{[2
]}

Sung, Yongjin ^{[3
]}

Kim, Kwanhyung ^{[4
]}

Dasari, Ramachandra R. ^{[3
]}

Feld, Michael S. ^{[3
]}

Choi, Wonshik ^{[1
]}

机构：

[1] Korea Univ, Dept Phys, Seoul 136701, South Korea

[2] Univ Penn, Dept Bioengn, Philadelphia, PA 19104 USA

[3] MIT, George R Harrison Spect Lab, Cambridge, MA 02139 USA

[4] LG Elect Inc, Seoul 137130, South Korea

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2012年 / 17卷 / 02期

基金：

美国国家科学基金会; 新加坡国家研究基金会; 美国国家卫生研究院;

关键词：

image processing; interferometry; microscopy; synthetic apertures; HOLOGRAPHIC MICROSCOPY; PHASE; LIMIT;

D O I：

10.1117/1.JBO.17.2.026003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image for arbitrary shearing direction and bias retardation. In addition, we obtain images at different depths without a scanning objective lens by numerically propagating the acquired coherent images. Our method achieves high-resolution and high-contrast 3-D DIC imaging of live biological cells. The proposed method will be useful for monitoring 3-D dynamics of intracellular particles. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.2.026003]

引用

页数：6

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