Gene expression profile of peripheral blood mononuclear cells in response to HIV-VLPs stimulation

被引：27
|
作者：
Buonaguro, Luigi [1 ,2 ]
Monaco, Alessandro [3 ]
Arico, Eleonora [5 ]
Wang, Ena [3 ]
Tornesello, Maria Lina [1 ,2 ]
Lewis, George K. [4 ]
Marincola, Franco M. [3 ]
Buonaguro, Franco M. [1 ,2 ]
机构：
[1] Ist Nazl Tumori Fond D Pascale, Lab Viral Oncogenesis & Immunotherapies, I-80131 Naples, Italy
[2] Ist Nazl Tumori Fond D Pascale, Dept Expt Oncol, AIDS Reference Ctr, I-80131 Naples, Italy
[3] NIH, Dept Transfus Med, Immunogenet Sect, Bethesda, MD 20892 USA
[4] Univ Maryland, Inst Human Virol, Sch Med, Baltimore, MD 21201 USA
[5] Ist Super Sanita, Dept Cell Biol & Neurosci, I-00161 Rome, Italy
来源：
BMC BIOINFORMATICS | 2008年 / 9卷 / Suppl 2期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1186/1471-2105-9-S2-S5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Background: Baculovirus-expressed HIV-1 Pr55gag Virus-Like Particles (HIV-VLPs) induce maturation and activation of monocyte-derived dendritic cells (MDDCs) with a production of Th1-and Th2-specific cytokines. Results: The analysis of genomic transcriptional profile of MDDCs, obtained from normal healthy donors and activated by HIV-VLPs, show the modulation of genes involved in the morphological and functional changes characterizing the MDDCs activation and maturation. Similar data are obtained using peripheral blood mononuclear cells (PBMCs), without further selection, showing the feasibility of a direct and "simplified" experimental procedure. Conclusions: The results here described show that the maturation pattern induced by HIV-VLPs in ex vivo generated MDDCs, can be observed also in CD14-expressing freshly derived PBMCs, with the possible identification of genetic predictors of individual response to immunogens.
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页数：7
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