16S rRNA metabarcoding unearths responses of rare gut microbiome of fathead minnows exposed to benzo[a]pyrene

被引:3
|
作者
DeBofsky, Abigail [1 ]
Xie, Yuwei [1 ]
Challis, Jonathan K. [1 ]
Ankley, Phillip J. [1 ]
Brinkmann, Markus [1 ,3 ]
Jones, Paul D. [1 ,3 ]
Giesy, John P. [1 ,2 ,4 ]
机构
[1] Univ Saskatchewan, Toxicol Ctr, Saskatoon, SK S7N 5B3, Canada
[2] Univ Saskatchewan, Dept Vet Biomed Sci, Saskatoon, SK, Canada
[3] Univ Saskatchewan, Sch Environm & Sustainabil, Saskatoon, SK, Canada
[4] Baylor Univ, Dept Environm Sci, Waco, TX 76798 USA
基金
加拿大自然科学与工程研究理事会;
关键词
Homeostasis; Next-generation sequencing; Freshwater fish; Persistence organics pollution; Molecular ecology; 16S metagenomics; POLYCYCLIC AROMATIC-HYDROCARBONS; FISH; COMMUNITIES; BIODIVERSITY; INFLAMMATION; DEGRADATION; WATERBORNE; PATHWAYS; BACTERIA; DIETARY;
D O I
10.1016/j.scitotenv.2021.151060
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Activities of gut microbiomes are often overlooked in assessments of ecotoxicological effects of environmental contaminants. Effects of the polycydic aromatic hydrocarbon, benzo[a]pyrene (BaP) on active gut microbiomes of juvenile fathead minnows (Pimephales promelas) were investigated. Fish were exposed for two weeks, to concentrations of 0, 1, 10, 100, or 1000 mu g BaPg(-1) in the diet. The active gut microbiome was characterized using 16S rRNA metabarcoding to determine its response to dietary exposure of BaP. BaP reduced alpha-diversity at the greatest exposure concentrations. Additionally, exposure to BaP altered community composition of active microbiome and resulted in differential proportion of taxa associated with hydrocarbon degradation and fish health. Neighborhood selection networks of active microbiomes were not reduced with greater concentrations of BaP, which suggests ecological resistance and/or resilience of gut microbiota. The active gut microbiome had a similar overall biodiversity as that of the genomic gut microbiota, but had a distinct composition from that of the 165 rDNA profile. Responses of alpha- and beta-diversities of the active microbiome to BaP exposure were consistent with that of genomic microbiomes. Normalized activity of microbiome via the ratio of rRNA to rDNA abundance revealed rare taxa that became active or dormant due to exposure to BaP. These differences highlight the need to assess both 165 rDNA and rRNA metabarcoding to fully derive bacterial compositional changes resulting from exposure to contaminants. (C) 2021 Elsevier B.V. All rights reserved.
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页数:10
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