The stress imposed on plants by soil flooding constitutes a major barrier to growth and productivity. The identification of soybean varieties that produce higher levels of isoflavones, is necessary as soybeans have been used as human food to reduce risks of chronic diseases. Thus, this study was conducted with the objective of quantifying proteins and isoflavones in soybean cultivars subjected to flooding at various stages of development. The cultivars 'BRS267', 'BRS257' and 'BRS213' were subjected to 15 days of flooding, starting at the stages V6 and V8 and 11 days under stress starting at the stage R4. The proteins in the grain were extracted and quantified and analyzed by SDS-PAGE electrophoresis. Isoflavones were extracted, separated and quantified on HPLC. The electrophoretic analysis of the three cultivars under study revealed the same pattern of banding relative to the total protein regardless of the treatment. However, it was noted that flooding led to an increase in the total contents of isoflavones in the BRS 267 plants flooded in stage R4, remaining constant in other cultivars.
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Poznan Univ Life Sci, Fac Food Sci & Nutr, Ul Wojska Polskiego 31, PL-60637 Poznan, PolandPoznan Univ Life Sci, Fac Food Sci & Nutr, Ul Wojska Polskiego 31, PL-60637 Poznan, Poland
Kuligowski, Maciej
Sobkowiak, Daria
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Poznan Univ Life Sci, Fac Food Sci & Nutr, Ul Wojska Polskiego 31, PL-60637 Poznan, PolandPoznan Univ Life Sci, Fac Food Sci & Nutr, Ul Wojska Polskiego 31, PL-60637 Poznan, Poland
Sobkowiak, Daria
Polanowska, Katarzyna
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Poznan Univ Life Sci, Fac Food Sci & Nutr, Ul Wojska Polskiego 31, PL-60637 Poznan, PolandPoznan Univ Life Sci, Fac Food Sci & Nutr, Ul Wojska Polskiego 31, PL-60637 Poznan, Poland
Polanowska, Katarzyna
Jasinska-Kuligowska, Iwona
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Poznan Univ Econ & Business, Inst Qual Sci, Dept Food Qual & Safety, Al Niepodleglosci 10, PL-61875 Poznan, PolandPoznan Univ Life Sci, Fac Food Sci & Nutr, Ul Wojska Polskiego 31, PL-60637 Poznan, Poland