Optical refocusing three-dimensional wide-field fluorescence lifetime imaging microscopy

被引：7
|
作者：
Wu, Qiang [1 ,2 ]
Guo, Shangyu [1 ,2 ]
Ma, Yinxing [1 ,2 ]
Gao, Feng [1 ,2 ]
Yang, Chengliang [1 ,2 ,3 ]
Yang, Ming [1 ,2 ]
Yu, Xuanyi [1 ,2 ]
Zhang, Xinzheng [1 ,2 ]
Rupp, Romano A. [1 ,2 ,4 ]
Xu, Jingjun [1 ,2 ,3 ]
机构：
[1] Nankai Univ, MOE Key Lab Weak Light Nonlinear Photon, TEDA Appl Phys Sch, Tianjin 300457, Peoples R China
[2] Nankai Univ, Sch Phys, Tianjin 300457, Peoples R China
[3] Chinese Acad Sci, State Key Lab Appl Opt, Changchun Inst Opt Fine Mech & Phys, Changchun 130033, Jinlin, Peoples R China
[4] Univ Vienna, Fac Phys, A-1090 Vienna, Austria
来源：
OPTICS EXPRESS | 2012年 / 20卷 / 02期
基金：
中国国家自然科学基金;
关键词：
SPECTRAL RESOLUTION;
D O I：
10.1364/OE.20.000960
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Three-dimensional fluorescence lifetime microscopy is achieved by combining wide-field fluorescence lifetime imaging with a remote optical refocusing method. As required for some applications in dynamic research for physics, chemistry, or biology, it is thereby not necessary to move the sample, i.e., the specimen is not disturbed during measurement. Using a fluorescent microsphere the performance of the system has been tested successfully with respect to three-dimensional fluorescence lifetime microscopy as well as time-resolved fluorescence spectroscopy. (C)2012 Optical Society of America
引用
收藏
页码：960 / 965
页数：6
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