Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector

被引：28
|
作者：
Mathew, Asha [1 ]
Cao, Hongliang [1 ]
Collin, Estelle [1 ]
Wang, Wenxin [1 ]
Pandit, Abhay [1 ]
机构：
[1] Natl Univ Ireland, Network Excellence Funct Biomat, Galway, Ireland
来源：
INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2012年 / 434卷 / 1-2期
关键词：
2-(Dimethylamino) ethyl methacrylate; Deactivation enhanced atom transfer radical polymerisation; Gene therapy; THERAPY; SYSTEMS; DNA; MICELLES; CARRIER; NEED; PEG;
D O I：
10.1016/j.ijpharm.2012.05.010
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
A unique hyperbranched polymeric system with a linear poly-2-dimethylaminoethyl methacrylate (pDMAEMA) block and a hyperbranched polyethylene glycol methyl ether methacrylate (PEGMEMA) and ethylene dimethacrylate (EGDMA) block was designed and synthesized via deactivation enhanced atom transfer radical polymerisation (DE-ATRP) for efficient gene delivery. Using this unique structure, with a linear pDMAEMA block, which efficiently binds to plasmid DNA (pDNA) and hyperbranched polyethylene glycol (PEG) based block as a protective shell, we were able to maintain high transfection levels without sacrificing cellular viability even at high doses. The transfection capability and cytotoxicity of the polymers over a range of pDNA concentration were analysed and the results were compared to commercially available transfection vectors such as polyethylene imine (branched PEI, 25 kDa), partially degraded poly(amido amine) dendrimer (dPAMAM; commercial name: SuperFect (R)) in fibroblasts and adipose tissue derived stem cells (ADSCs). (c) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：99 / 105
页数：7
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