The Effect of Three Variants of Extracting Solvents on the Total Phenolic Content and Antioxidant Activity of Diospyros Blancoi Seeds

In this study, the effects of methanol, acetone, and ethyl acetate solvents on total phenolic content and antioxidant activity in Diospyros blancoi seeds extract were examined. Maceration extraction technique was selected in extracting the seeds. Determination of total phenolic content (TPC) and antioxidant activity used Folin-Ciocalteu and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. Radical scavenging activity data were interpreted from the calculations of the half maximal inhibitory concentration (IC50) values. TPC was found to be higher (603.43 +/- 0.75 mg gallic acid equivalent/g dry powder) in D. blancoi seeds in the acetone extract compared to the methanol and ethyl acetate extracts. The highest antioxidant activity for D. blancoi seeds extracts was in methanol solvent (IC50: 2.72 mu g/mL), with IC50 of ascorbic acid as a positive control was 3.34 mu g/mL. These results indicate that TPC contributes to antioxidant activity in D. blancoi seeds.