Metabolite and Transcriptome Profiling Analysis Revealed That Melatonin Positively Regulates Floral Scent Production in Hedychium coronarium

被引:18
|
作者
Abbas, Farhat [1 ]
Zhou, Yiwei [1 ]
He, Jingjuan [1 ]
Ke, Yanguo [1 ,2 ]
Qin, Wang [1 ]
Yu, Rangcai [3 ]
Fan, Yanping [1 ,4 ]
机构
[1] South China Agr Univ, Coll Forestry & Landscape Architecture, Res Ctr Ornamental Plants, Guangzhou, Peoples R China
[2] Kunming Univ, Coll Econom & Management, Kunming, Peoples R China
[3] South China Agr Univ, Coll Life Sci, Guangzhou, Peoples R China
[4] South China Agr Univ, Guangdong Key Lab Innovat Dev & Utilizat Forest P, Guangzhou, Peoples R China
来源
FRONTIERS IN PLANT SCIENCE | 2021年 / 12卷
基金
中国国家自然科学基金;
关键词
Hedychium coronarium; metabolome; transcriptome; melatonin; floral scent; PLANT VOLATILES; FRAGRANCE BIOSYNTHESIS; QUANTITATIVE-ANALYSES; FLOWER SCENT; EXPRESSION; TERPENOIDS; DIVERSITY; SYNTHASES; NETWORKS; GENOMICS;
D O I
10.3389/fpls.2021.808899
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Melatonin is a pleiotropic molecule that regulates a variety of developmental processes. Floral volatiles are important features of flowers that facilitate flower-visitor interactions by attracting pollinators, structure flower-visitor communities, and play defensive roles against plant and flower antagonists. Aside from their role in plants, floral volatiles are an essential ingredient in cosmetics, perfumes, pharmaceuticals, and flavorings. Herein, integrated metabolomic and transcriptomic approaches were carried out to analyze the changes triggered by melatonin exposure during the Hedychium coronarium flower development stages. Quantitative analysis of the volatiles of H. coronarium flowers revealed that volatile organic compound emission was significantly enhanced after melatonin exposure during the half bloom (HS), full bloom (FB) and fade stage (FS). Under the melatonin treatment, the emission of volatile contents was highest during the full bloom stage of the flower. Variable importance in projection (VIP) analysis and partial least-squares discriminant analysis (PLS-DA) identified 15 volatile compounds with VIP > 1 that were prominently altered by the melatonin treatments. According to the transcriptome sequencing data of the HS, FB, and FS of the flowers, 1,372, 1,510, and 1,488 differentially expressed genes were identified between CK-HS and 100MT-HS, CK-FB and 100MT-FB, and CK-FS and 100MT-FS, respectively. Among the significant differentially expressed genes (DEGs), 76 were significantly upregulated and directly involved in the floral scent biosynthesis process. In addition, certain volatile organic compounds were substantially linked with various DEGs after combining the metabolome and transcriptome datasets. Moreover, some transcription factors, such as MYB and bHLH, were also significantly upregulated in the comparison, which might be related to the floral aroma mechanism. Our results suggested that melatonin increased floral aroma production in H. coronarium flowers by modifying the expression level of genes involved in the floral scent biosynthesis pathway. These findings serve as a foundation for future research into the molecular mechanisms underlying the dynamic changes in volatile contents induced by melatonin treatment in H. coronarium.
引用
收藏
页数:18
相关论文
共 14 条
  • [1] Transcriptome profiling provides new insights into the formation of floral scent in Hedychium coronarium
    Yuechong Yue
    Rangcai Yu
    Yanping Fan
    BMC Genomics, 16
  • [2] Transcriptome profiling provides new insights into the formation of floral scent in Hedychium coronarium
    Yue, Yuechong
    Yu, Rangcai
    Fan, Yanping
    BMC GENOMICS, 2015, 16
  • [3] Genome-Wide Analysis Reveals the Potential Role of MYB Transcription Factors in Floral Scent Formation in Hedychium coronarium
    Abbas, Farhat
    Ke, Yanguo
    Zhou, Yiwei
    Yu, Yunyi
    Waseem, Muhammad
    Ashraf, Umair
    Wang, Chutian
    Wang, Xiaoyu
    Li, Xinyue
    Yue, Yuechong
    Yu, Rangcai
    Fan, Yanping
    FRONTIERS IN PLANT SCIENCE, 2021, 12
  • [4] Genome-Wide Analysis and Characterization of the Aux/IAA Family Genes Related to Floral Scent Formation in Hedychium coronarium
    Ke, Yanguo
    Abbas, Farhat
    Zhou, Yiwei
    Yu, Rangcai
    Yue, Yuechong
    Li, Xinyue
    Yu, Yunyi
    Fan, Yanping
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019, 20 (13)
  • [5] Coordinated and High-Level Expression of Biosynthetic Pathway Genes Is Responsible for the Production of a Major Floral Scent Compound Methyl Benzoate in Hedychium coronarium
    Yue, Yuechong
    Wang, Lan
    Yu, Rangcai
    Chen, Feng
    He, Jieling
    Li, Xinyue
    Yu, Yunyi
    Fan, Yanping
    FRONTIERS IN PLANT SCIENCE, 2021, 12
  • [6] SCARECROW-like GRAS protein PES positively regulates petunia floral scent production
    Shor, Ekaterina
    Ravid, Jasmin
    Sharon, Elad
    Skaliter, Oded
    Masci, Tania
    Vainstein, Alexander
    PLANT PHYSIOLOGY, 2023, 192 (01) : 409 - 425
  • [7] Transcriptome analysis of thermogenic Arum concinnatum reveals the molecular components of floral scent production
    Onda, Yoshihiko
    Mochida, Keiichi
    Yoshida, Takuhiro
    Sakurai, Tetsuya
    Seymour, Roger S.
    Umekawa, Yui
    Pirintsos, Stergios Arg
    Shinozaki, Kazuo
    Ito, Kikukatsu
    SCIENTIFIC REPORTS, 2015, 5
  • [8] Transcriptome analysis of thermogenic Arum concinnatum reveals the molecular components of floral scent production
    Yoshihiko Onda
    Keiichi Mochida
    Takuhiro Yoshida
    Tetsuya Sakurai
    Roger S. Seymour
    Yui Umekawa
    Stergios Arg Pirintsos
    Kazuo Shinozaki
    Kikukatsu Ito
    Scientific Reports, 5
  • [9] Transcriptome-Based WGCNA Analysis Reveals Regulated Metabolite Fluxes between Floral Color and Scent in Narcissus tazetta Flower
    Yang, Jingwen
    Ren, Yujun
    Zhang, Deyu
    Chen, Xuewei
    Huang, Jiazhi
    Xu, Yun
    Aucapina, Cristina Belen
    Zhang, Yu
    Miao, Ying
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021, 22 (15)
  • [10] Metabolite Profiling and Transcriptome Analysis Revealed the Chemical Contributions of Tea Trichomes to Tea Flavors and Tea Plant Defenses
    Li, Penghui
    Xu, Yujie
    Zhang, Yanrui
    Fu, Jiamin
    Yu, Shuwei
    Guo, Huimin
    Chen, Zhihui
    Chen, Changsong
    Yang, Xiaogen
    Wang, Shucai
    Zhao, Jian
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2020, 68 (41) : 11389 - 11401