Evaluation of the Merits of the New Method of Oviductus Ranae by HPLC-DAD

In this study, a new method of evaluating the merits of Oviductus Ranae has been established, and HPLC was used to determine the contents of 4-cholesten-3-one, 3 beta-hydroxy-cholesta-5-en-7-one, and cholesterol in Oviductus Ranae at the same time. The determination was achieved on a Waters Xbridge-C-18 (4.6x250mm, 5 mu m) column maintained at a temperature of 30 degrees C, using methanol-water (90:10%, v/v) as the mobile phase with the flow rate of 1.0mL/min and detected using UV-Visible detector at a UV wavelength of 248nm. Detection was carried out at the wavelength of 220nm and 240nm with the sample volume of 10 uL. The 4-cholesten-3-one, 3 beta-hydroxy- cholesta-5-en-7-one, and cholesterol in Oviductus Ranae of the analytical samples represent good liner relations in the range of 0.0030150-0.010050 mu g, 0.003042-0.010140 mu g, and 0.6126-2.0420 mu g, respectively. The average recovery of hree samples were 98.8%, RSD=0.48% (n=6); 99.4%, RSD=1.51% (n=6); 100.2%, RSD=1.2% (n=6), respectively, and showed good stability, precision, and repeatability. This method can determine the contents of 4-cholesten-3-one, 3 beta-hydroxy-cholesta-5-en-7-one, and cholesterol in Oviductus Ranae at the same time and was proven to be fast, accurate, and stable.