Mechanosensitive channel functions to alleviate the cell lysis of marine bacterium, Vibrio alginolyticus, by osmotic downshock

被引：33

作者：

Nakamaru, Y ^{[1
]}

Takahashi, Y ^{[1
]}

Unemoto, T ^{[1
]}

Nakamura, T ^{[1
]}

机构：

[1] Chiba Univ, Fac Pharmaceut Sci, Lab Membrane Biochem, Inage Ku, Chiba 2638522, Japan

来源：

FEBS LETTERS | 1999年 / 444卷 / 2-3期

关键词：

mechanosensitive channel with large conductance; osmotic lysis; marine bacterium; Vibrio alginolyticus;

D O I：

10.1016/S0014-5793(99)00054-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mechanosensitive channel with large conductance of Escherichia coli is the first to be cloned among stretch-activated channels. Although its activity was characterized by a patch clamp method, a physiological role of the channel has not been proved. The marine bacterium, Vibrio alginolyticus, is sensitive to osmotic stress and cell lysis occurs under osmotic downshock. We introduced an mscL. gene into Vibrio alginolyticus, and the mechanosensitive channel with large conductance functions was found to alleviate cell lysis by osmotic downshock, This is the first report to show a physiological role of the mechanosensitive channel with large conductance, (C) 1999 Federation of European Biochemical Societies.

引用

页码：170 / 172

页数：3

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