Recovering and reamplifying of the differentially expressed cDNA bands isolated from mRNA differential display - A modified method

被引：7
|
作者：
Wang, H
Xia, ZB
Wang, YH
Li, CH
Sun, LY
机构：
[1] Inst Basic Med Sci, Dept Tumor Mol Biol, Beijing 100850, Peoples R China
[2] Tai Ping Hosp, Beijing 100850, Peoples R China
[3] Inst Radiat Med, Beijing 100850, Peoples R China
[4] Henal Med Univ, Dept Pathol, Zhengzhou 40052, Peoples R China
来源：
MOLECULAR BIOTECHNOLOGY | 1998年 / 9卷 / 02期
关键词：
mRNA differential display; differentially expressed cDNA bands; retrieving (or recovering) and reamplifying;
D O I：
10.1007/BF02760818
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Methods for retrieving and reamplifying the differentially expressed cDNA bands have been modified. Direct reamplification of differentially expressed bands after cutting from a polyacrylamide gel (PAG) followed by a simple rinse and crush step has proved to be more convenient and effective than the traditional glycogen-precipitation method. Combination of 30 cycles of differential display (DD) polymerase chain reaction (PCR) and 20 cycles of standard PCR reaction also yielded higher reamplification rates.
引用
收藏
页码：171 / 173
页数：3
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