Isolation, cloning and characterization of Polistes dominulus venom phospholipase A1 and its isoforms

被引:13
|
作者
Moawad, TI [1 ]
Hoffman, DR
Zalat, S
机构
[1] Suez Canal Univ, Fac Sci, Dept Zool, Ismailia, Egypt
[2] E Carolina Univ, Dept Pathol & Lab Med, Brody Sch Med, Greenville, NC 27858 USA
来源
ACTA BIOLOGICA HUNGARICA | 2005年 / 56卷 / 3-4期
关键词
Hymenoptera; Vespidae; Polistinae; Polistes dominulus; venom phospholipase A1;
D O I
10.1556/ABiol.56.2005.3-4.9
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Polistes dominithis is known to be an allergenically important social wasp. Its venom has four major allergens (ziv. phospholipase A1, hyaluronidase, antigen 5 and a serine-protease). Amino acid sequences of its serine-protease and Antigen 5 have been published. In this paper, the partial amino acid sequence of its venom phospholipase native protein is reported. Also, we give an account to the complete nucleotide sequence of Polistes domiulus venom phospholipase Al gene, its isoforms and their complete deduced amino acid sequences. Their similarity to the other phospholipases Al of the family: Vespidae is discussed.
引用
收藏
页码:261 / 274
页数:14
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