Real-time quantum cascade laser-based infrared microspectroscopy in-vivo

被引：2
|
作者：
Kroeger-Lui, N. [1 ]
Haase, K. [1 ]
Pucci, A. [1 ]
Schoenhals, A. [1 ]
Petrich, W. [1 ]
机构：
[1] Kirchhoff Inst Phys, Neuenheimer Feld 227, D-69120 Heidelberg, Germany
来源：
BIOMEDICAL VIBRATIONAL SPECTROSCOPY 2016: ADVANCES IN RESEARCH AND INDUSTRY | 2016年 / 9704卷
关键词：
Quantum cascade laser; real-time; infrared; microscopy; microorganism; fermentation; LIVE CELLS; SPECTROSCOPY; FREQUENCY;
D O I：
10.1117/12.2213239
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Infrared microscopy can be performed to observe dynamic processes on a microscopic scale. Fourier-transform infrared spectroscopy-based microscopes are bound to limitations regarding time resolution, which hampers their potential for imaging fast moving systems. In this manuscript we present a quantum cascade laser-based infrared microscope which overcomes these limitations and readily achieves standard video frame rates. The capabilities of our setup are demonstrated by observing dynamical processes at their specific time scales: fermentation, slow moving Amoeba Proteus and fast moving Caenorhabditis elegans. Mid-infrared sampling rates between 30 min and 20 ms are demonstrated.
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页数：8
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