Sphingomonas aeria sp nov., isolated from air

A Gram-negative, aerobic, non-spore-forming, non-motile, yellow-pigmented and rod-shaped bacterial strain, designated B093034(T), was isolated from air at the foot of Xiangshan mountain, located in Beijing, China. Cells of strain B093034(T) were oxidase-negative and catalase-positive. Growth was observed at 4-41 degrees C, at pH 4.5-10.0 and at 0-7% (w/v) NaCl. The isolate contained Q-10 as the predominant isoprenoid quinone, summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c), C-16:0 and C(14:0)2-OH as the major fatty acids, sym-homospermidine as the major polyamine, and sphingoglycolipid, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, diphosphatidylglycerol, aminolipid, two unidentified phospholipids and three unidentified polar lipids as the polar lipids. The DNA G+C content was 67.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain B093034(T) grouped with members of the genus Sphingomonas and was closely related to Sphingomonas sanguinis IFO 13937(T) (96.49% similarity), Sphingomonas pseudosanguinis G1-2(T) (96.37 %), Sphingomonas ginsenosidimutans Gsoil 1429(T) (95.99 %) and Sphingomonas endophytica YIM 65583(T) (95.78 %). On the basis of the polyphasic evidence presented here, strain B093034(T) represents a novel species of the genus Sphingomonas, for which the name Sphingomonas aeria sp. nov. is proposed. The type strain is B093034(T) (=CFCC 13949(T)=LMG 30133(T)).