Preparation of core-shell Fe3O4/SiO2 microspheres as adsorbents for purification of DNA

被引：21
|
作者：
Gai, Ligang [1 ]
Li, Zhili [1 ]
Hou, Yunhua [2 ]
Jiang, Haihui [1 ]
Han, Xiaoyun [1 ]
Ma, Wanyong [1 ]
机构：
[1] Shandong Inst Light Ind, Sch Chem Engn, Shandong Prov Key Lab Fine Chem, Jinan 250353, Peoples R China
[2] Shandong Inst Light Ind, Sch Food Sci & Bioengn, Shandong Prov Key Lab Microbial Engn, Jinan 250353, Peoples R China
来源：
JOURNAL OF PHYSICS D-APPLIED PHYSICS | 2010年 / 43卷 / 44期
关键词：
MAGNETIC SILICA MICROSPHERES; MONTE-CARLO SIMULATIONS; MOLECULAR-BEAM EPITAXY; PLASMID DNA; SUPERPARAMAGNETIC NANOPARTICLES; BACTERIAL-CELLS; GENOMIC DNA; GIANT DNA; IRON; ADSORPTION;
D O I：
10.1088/0022-3727/43/44/445001
中图分类号：
O59 [应用物理学];
学科分类号：
摘要：
Nearly monodisperse core-shell Fe3O4/SiO2 microspheres have been prepared via a glycol reduction method followed by a modified Stober process. The thickness of the silica shells can be tuned in the range 33-53 nm by varying the amount of tetraethyl silicate (TEOS) during syntheses. The magnetic composite microspheres were characterized with XRD, XPS, FTIR, TEM, ICP-OES and VSM, and further tested as adsorbents for purification of plasmid DNA from Escherichia coli DII5 alpha cells. The magnetic purification of plasmid DNA leads to satisfying integrity, yield and purity in comparison with those isolated by the traditional phenol-chloroform extraction.
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页数：8
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