Genotypic and phenotypic characterization of Pseudomonas aeruginosa isolates from urinary tract infections

被引：38
|
作者：
Tielen, Petra [1 ]
Narten, Maike [1 ]
Rosin, Nathalie [1 ]
Biegler, Ilona [1 ]
Haddad, Isam [1 ]
Hogardt, Michael [2 ]
Neubauer, Ruediger [3 ,4 ]
Schobert, Max [1 ]
Wiehlmann, Lutz [4 ]
Jahn, Dieter [1 ]
机构：
[1] Tech Univ Carolo Wilhelmina Braunschweig, Inst Microbiol, D-38106 Braunschweig, Germany
[2] Max von Pettenkofer Inst Hyg & Med Microbiol, D-81377 Munich, Germany
[3] Phys Ctr Urinary Tract Dis, D-34123 Kassel, Germany
[4] Med Sch Hanover, Clin Res Grp, D-30625 Hannover, Germany
来源：
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY | 2011年 / 301卷 / 04期
关键词：
SNP chip typing; LPS typing; Virulence factors; Biofilm formation; CYSTIC-FIBROSIS PATIENTS; VIRULENCE FACTORS; GENETIC ADAPTATION; TWITCHING MOTILITY; BIOFILM FORMATION; GENOME DIVERSITY; STRAINS; PATHOGENESIS; DEFICIENT; ALGINATE;
D O I：
10.1016/j.ijmm.2010.10.005
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Pseudomonas aeruginosa is one of the most frequent agents of urinary tract infections especially in patients with indwelling urethral catheters. A total of 30 P. aeruginosa isolates from urinary tract infections was investigated for their genotypic and phenotypic characteristics. 'Single Nucleotide Polymorphism' chip typing experiments in combination with bioinformatical cluster analyses allowed genotypic grouping of the isolates. Some similarities to strains from lung infections but also to environmental strains were observed. Finally, several urinary tract-specific groups were identified indicating a strong heterogeneity of the urethral isolates. Pyoverdin, protease, and phospholipase A production in combination with quorum sensing activity and biofilm formation were common phenotypic characteristics of these strains. In contrast, swarming phenotypes, the production of pyocyanin, and the extracellular enzymes phospholipase C and elastase were rarely observed. Interestingly, strains isolated from catheter-associated infections showed significantly enhanced biofilm formation, decreased motility, and a slightly increased expression of virulence factors in relation to isolates from acute urinary tract infections. (C) 2010 Elsevier GmbH. All rights reserved.
引用
收藏
页码：282 / 292
页数：11
相关论文
共 50 条
  • [1] Phenotypic and genotypic detection of antibiotic resistance of Pseudomonas aeruginosa isolated from urinary tract infections
    Abbas, Hisham A.
    El-Ganiny, Amira M.
    Kamel, Hend A.
    [J]. AFRICAN HEALTH SCIENCES, 2018, 18 (01) : 11 - 21
  • [2] Antimicrobial Susceptibilities and Clinical Characterization of Pseudomonas aeruginosa Isolates from Urinary Tract Infections
    Zhang, Xiaobing
    Niu, Siqiang
    Zhang, Liping
    [J]. UROLOGIA INTERNATIONALIS, 2014, 93 (04) : 464 - 469
  • [3] Phenotypic and genomic characterization of Pseudomonas aeruginosa isolates recovered from catheter-associated urinary tract infections in an Egyptian hospital
    Eladawy, Mohamed
    Thomas, Jonathan C.
    Hoyles, Lesley
    [J]. MICROBIAL GENOMICS, 2023, 9 (10):
  • [4] Characterization of Pseudomonas aeruginosa isolates from patients with urinary tract infections during antibiotic therapy
    Horii, T
    Muramatsu, H
    Morita, M
    Maekawa, M
    [J]. MICROBIAL DRUG RESISTANCE, 2003, 9 (02) : 223 - 229
  • [5] Phenotypic and genotypic within-host diversity of Pseudomonas aeruginosa urinary isolates
    Agnès Cottalorda
    Sandrine Dahyot
    Anaïs Soares
    Kevin Alexandre
    Isabelle Zorgniotti
    Manuel Etienne
    Estelle Jumas-Bilak
    Martine Pestel-Caron
    [J]. Scientific Reports, 12
  • [6] Phenotypic and genotypic within-host diversity of Pseudomonas aeruginosa urinary isolates
    Cottalorda, Agnes
    Dahyot, Sandrine
    Soares, Anais
    Alexandre, Kevin
    Zorgniotti, Isabelle
    Etienne, Manuel
    Jumas-Bilak, Estelle
    Pestel-Caron, Martine
    [J]. SCIENTIFIC REPORTS, 2022, 12 (01)
  • [7] Genotypic and phenotypic characterization of Pseudomonas aeruginosa isolates from post-cataract endophthalmitis patients
    Priya, Jeganathan Lakshmi
    Prajna, Lalitha
    Mohankumar, Vidyarani
    [J]. MICROBIAL PATHOGENESIS, 2015, 78 : 67 - 73
  • [8] Phenotypic and genotypic characterization of clinical Pseudomonas aeruginosa
    Mallikarjuna, Pedduru, V
    Dhanashree, Biranthabail
    [J]. JOURNAL OF TAIBAH UNIVERSITY MEDICAL SCIENCES, 2023, 18 (03): : 480 - 487
  • [9] Genotypic and Phenotypic Characterization of MBL Genes in Pseudomonas aeruginosa Isolates from the Non-hospital Environment
    Ejikeugwu, Chika
    Esimone, Charles
    Iroha, Ifeanyichukwu
    Eze, Peter
    Ugwu, Malachy
    Adikwu, Michael
    [J]. JOURNAL OF PURE AND APPLIED MICROBIOLOGY, 2018, 12 (04): : 1877 - 1885
  • [10] Evaluation of phenotypic and genotypic methods for the identification and characterization of bacterial isolates recovered from catheter-associated urinary tract infections
    Varney, Adam M.
    Mannix-Fisher, Eden
    Thomas, Jonathan C.
    McLean, Samantha
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2024, 135 (07)
12345