Cloning, sequencing and expression of human manganese superoxide dismutase gene cDNA

被引：0

作者：

Luo, XY ^{[1
]}

Zhou, LJ ^{[1
]}

Chen, XS ^{[1
]}

Wang, YX ^{[1
]}

Xie, BT ^{[1
]}

Wang, JY ^{[1
]}

Chen, YP ^{[1
]}

机构：

[1] NAVAL GEN HOSP, BEIJING 100037, PEOPLES R CHINA

来源：

PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS | 1996年 / 23卷 / 04期

关键词：

manganese superoxide dismutase; RT-PCR; gene cloning;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cDNA encoding human manganese superoxide dismutase was amplified from the human liver total RNA by RT-PCR, ligated into pBV220. The cloned gene was analyzed by restriction enzymes EcoR I, Sal I, and BamH I. The sequence of the cloned gene was determined. hMnSOD was induced and expressed upon temperature shift from 32 degrees C to 42 degrees C. Mn2+ supplementation in the bacterial growth media resulted in about 3-fold increasing of SOD activity. The special protein expressed accounts for 14% of the total protein of the bacteria.

引用

页码：349 / 352

页数：4

共 8 条

[1] HUMAN MN SUPEROXIDE-DISMUTASE CDNA SEQUENCE
BECK, Y
OREN, R
AMIT, B
LEVANON, A
GORECKI, M
HARTMAN, JR
[J]. NUCLEIC ACIDS RESEARCH, 1987, 15 (21) : 9076 - 9076
[2] INCREASED MANGANESE SUPEROXIDE-DISMUTASE EXPRESSION SUPPRESSES THE MALIGNANT PHENOTYPE OF HUMAN-MELANOMA CELLS
CHURCH, SL
GRANT, JW
RIDNOUR, LA
OBERLEY, LW
SWANSON, PE
MELTZER, PS
TRENT, JM
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (07) : 3113 - 3117
[3] DAVID AM, 1991, BIOTECHNOLOGY, V9, P637
[4] RECOMBINANT HUMAN SUPEROXIDE DISMUTASES - PRODUCTION AND POTENTIAL THERAPEUTIC USES
GORECKI, M
BECK, Y
HARTMAN, JR
FISCHER, M
WEISS, L
TOCHNER, Z
SLAVIN, S
NIMROD, A
[J]. FREE RADICAL RESEARCH COMMUNICATIONS, 1991, 12-3 : 401 - 410
[5] HUBER W, 1981, EUR J RHEUMATOL INFL, V4, P173
[6] MCCORD JM, 1985, NEW ENGL J MED, V312, P159
[7] SUPEROXIDE-DISMUTASE - PHOTOCHEMICAL AUGMENTATION ASSAY
MISRA, HP
FRIDOVICH, I
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1977, 181 (01) : 308 - 312
[8] OBERLEY LW, 1979, CANCER RES, V39, P1141

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