Streptomyces matensis laminaripentaose hydrolase is an 'inverting' β-1,3-glucanase

被引：14

作者：

Nishimura, T

Bignon, C

Allouch, J

Czjzek, M

Darbon, H

Watanabe, T

Henrissat, B

机构：

[1] Univ Aix Marseille 1, CNRS, F-13402 Marseille 20, France

[2] Univ Aix Marseille 2, CNRS, F-13402 Marseille 20, France

[3] Niigata Univ, Fac Agr, Dept Appl Biol Chem, Niigata 95021, Japan

来源：

FEBS LETTERS | 2001年 / 499卷 / 1-2期

关键词：

beta-1,3-glucanase; laminaripentaose hydrolase; reaction mechanism; Streptomyces matensis;

D O I：

10.1016/S0014-5793(01)02551-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The laminaripentaose-producing beta -1,3-glucanase of Streptomyces matensis is a member of the glycoside hydrolase family GH-64. We have constructed and purified a recombinant hexahistidine-tagged form of the enzyme for characterisation, The enzyme, which exists as a monomer in solution, hydrolysis beta -1,3-glucan by a mechanism leading to overall inversion of the anomeric configuration. This is the first determination of the mechanism prevailing in glycoside hydrolase family GH-64 and this is the first characterisation of an 'inverting' beta -1,3-glucanase. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B,V, All rights reserved.

引用

页码：187 / 190

页数：4

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