Synthesis of tritium labeled [3R-3H]-, and [3S-3H]-L-phenylalanine

被引：6

作者：

Jemielity, J ^{[1
]}

Kaski, R ^{[1
]}

Kaska, M ^{[1
]}

机构：

[1] Univ Warsaw, Dept Chem, PL-02089 Warsaw, Poland

来源：

JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS | 2001年 / 44卷 / 04期

关键词：

tritium; labeling; optical isotopomer; cinnamic acid; phenylalanine; enzyme;

D O I：

10.1002/jlcr.461

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthesis of two selectively labeled isotopomers of L-phenylalanine, (Phe), using chemical and enzymatic methods is reported. The [3R-H-3]-L-Phe isotopomer has been obtained from [3-H-3]cinnamic acid prepared from benzaldehyde and malonic acid using tritiated water as a source of radioactive label, and by addition of ammonia in the presence of enzyme PAL. The [3S-H-3]-L-Phe isotopomer has been synthesized by addition of ammonia to cinnamic acid in a buffered medium containing PAL and tritiated water.

引用

页码：295 / 304

页数：10

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