Development of a sensitive gene expression reporter system and an inducible promoter-repressor system for Clostridium acetobutylicum

被引：54
|
作者：
Girbal, L
Mortier-Barrière, I
Raynaud, F
Rouanet, C
Croux, C
Soucaille, P
机构：
[1] CNRS, Ctr Bioingn Gilbert Durand, Lab Biotechnol Bioprocedes,UMR INRA 792, UMR 5504, F-31077 Toulouse 4, France
[2] CRITT Bioind, CRT, INSA, DGBA, F-31077 Toulouse, France
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2003年 / 69卷 / 08期
关键词：
D O I：
10.1128/AEM.69.8.4985-4988.2003
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A sensitive gene expression reporter system was developed for Clostridium acetobutylicum ATCC 824 by using a customized gusA expression cassette. In discontinuous cultures, time course profiles of beta-glucuronidase specific activity reflected adequately in vivo dynamic up- and down-regulation of acidogenesis- and/or solventogenesis-associated promoter expression in C. acetobutylicum. Furthermore, a new inducible gene expression system was developed in C. acetobutylicum, based on the Staphylococcus xylosus xylose operon promoter-repressor regulatory system.
引用
收藏
页码：4985 / 4988
页数：4
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