The aims of this study were to develop a serum plate agglutination test (LAT) antigen and microagglutination (MAT) test antigen for the serologic detection of turkeys that have been exposed to B. avium with respiratory disease symptom and to compare sensitivity of commercial enzyme-linked immunosorbent assay (ELISA) with these tests. All serum samples were collected from commercial turkey enterprises in Izmir, samples from 900 turkeys with symptoms of respiratory disease constituted the material of this study. The seroprevalence was determined as 21.6 %, 13.8 %, 12.1 % for ELISA, LAT, and MAT tests, respectively. As a conclusion, although ELISA was defined as the most sensitive test for screening of B. avium antibodies with this study, LAT and MAT's could also be used for this purpose.