Synthesis of Copper Oxide Nanoparticles (CuO-NPs) and its Evaluation of Antibacterial Activity Against P. aeruginosa Biofilm Gene's

Copper Oxide Nanoparticles (CuO-NPs) were successfully synthesized using sol-gel method Pseudomonas aeruginosa is an opportunistic bacterial pathogen in different disease as Diabetic Foot ulcer. Present study Focused on virulence gene as specific gene which increase the ability of P. aeruginosa to produce biofilms. Biofilm is an important Features for chronic colonization of Inflamed bacteria. From Diabetic Foot Patients ulcer isolated this bacteria, after diagnosing by bacterial method, sequencing for Elf C gene as specific virulence genes was studied in Iraqi hospital, P. aeruginosa consider as a chronic colonization in foot ulcer, therefore there is a real needing to suggest an alternative treatment materials effect on virulence genes. CuO-NPs became accepted solution used as an alternative to antibiotics. The effect of Copper Oxide nanoparticles on the genetic variation of one of virulence gene which is Elf C gene before treatment and after it, that was evaluated as the aim of our study. Present results illustrated increasing deletion effect for CuO-NPs on Cytosine nitrogen base which consider as evolution steps among genetic variation.