Cross Species Transfer of Microsatellite Loci in Scolytidae Species Mostly Associated with Mango (Mangifera indica L., Anacardiaceae) Quick Decline Disease

Mango bark beetle, Hypocryphalus mangiferae Stebbing (Coleoptera: Curculionidae: Scolytinae) is frequently associated with mango quick decline disease in Pakistan, since it is also reported as a potential vector of quick decline disease. Hereby, we report the cross-amplification of five microsatellite markers in Hypocryphalus mangiferae, which were primarily characterized for other Scolytidae species. The markers were tested on 45 mango bark beetles individuals from nine different locations of Pakistan. Allelic richness ranged from two to eight alleles, the observed heterozygosity from 0.03 to 0.67, and the expected heterozygosity from 0.10 to 0.70. Two loci showed significant deviation from Hardy-Weinberg equilibrium and no linkage between the loci was detected. We also investigated cross-species amplification in Xyleborus spp. using the same five microsatellites. Three of the loci were monomorphic and two showed three and two alleles, respectively. Observed heterozygosity values ranged from 0.3824 to 0.0882, those for expected heterozygosity from 0. to 0.2752. These markers could potentially contribute in the management of the mango bark beetle.