Monoclonal antibodies directed against lipopolysaccharide of Legionella pneumophila serogroup 1

被引：0
|
作者：
Baranov, AM [1 ]
Vetchinin, SS [1 ]
Baranova, EV [1 ]
Biketov, SF [1 ]
Svetoch, EA [1 ]
机构：
[1] State Res Ctr Appl Microbiol, Obolensk 142279, Moscow Region, Russia
来源：
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1998年 / 45卷 / 06期
关键词：
Legionella pneumophila; monoclonal antibodies;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The monoclonal antibodies (MAbs) against lipopolysaccharide of virulent strain of Legionella pneumophila serogroup 1 were produced. Three most productive hybrid clones (5F4, 5F10 and 2C9) were selected from fusions of mouse myeloma cells with spleen cells from BALB/c mice, immunized with bacterial outer membrane antigens. All generated clones were IgG-secreting. The MAbs had narrow strain specificity and showed no cross-reactions with other unrelated bacterial species. These antibodies were tested in sandwich ELISA. The results suggest that the MAbs could be used for diagnostic purposes.
引用
收藏
页码：1163 / 1168
页数：6
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