Accurate determination of process variables in a solid-state fermentation system

被引：34

作者：

Smits, JP ^{[1
]}

Rinzema, A ^{[1
]}

Tramper, J ^{[1
]}

Schlosser, EE ^{[1
]}

Knol, W ^{[1
]}

机构：

[1] AGR UNIV WAGENINGEN, DEPT FOOD SCI, FOOD & BIOPROC ENGN GRP, NL-6700 EV WAGENINGEN, NETHERLANDS

来源：

PROCESS BIOCHEMISTRY | 1996年 / 31卷 / 07期

关键词：

D O I：

10.1016/S0032-9592(96)00019-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The solid-stare fermentation (SSF) method described enabled accurate determination of variables related to biological activity. Growth, respiratory activity and production of carboxymethyl-cellulose-hydrolysing enzyme (CMC-ase) activity by Trichoderma reesei QM9414 on wheat bran was used as a model SSF. The standard deviation (s) of measured substrate weight loss and glucosamine content, and the overall standard deviation of oxygen consumption rate (OCR) and carbon-dioxide production rate (CPR) after 72 h of fermentation were less than 7% of the mean measured values. A statistical method to estimate the standard deviation of time-dependent data is presented. A closed carbon balance could be set up with the results of the measurements of CPR, elemental compostion and chemical composition, indicating the reliability of the methods used. Measurements of CMC-ase activity were less accurate due to interaction between substrate and product. The measurement of the ATP level was also less accurate. Copyright (C) 1996 Elsevier Science

引用

页码：669 / 678

页数：10

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