DIVERSITY OF IRANIAN STRAINS OF PSEUDOMONAS SYRINGAE pv. SYRINGAE AND PSEUDOMONAS SYRINGAE pv. LACHRYMANS

被引：0

作者：

Izadiyan, M. ^{[1
]}

Taghavi, S. M. ^{[1
]}

机构：

[1] Shiraz Univ, Coll Agr, Dept Plant Protect, Shiraz, Iran

来源：

JOURNAL OF PLANT PATHOLOGY | 2017年 / 99卷 / 02期

关键词：

genetic variability; P; syringae; pathovars; IS50-PCR; RAPD-PCR; FRAGMENT LENGTH POLYMORPHISM; GENETIC DIVERSITY; RAPD-PCR; DISEASE; PATHOGENICITY; VIRULENCE; HOSTS; REP;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Strains of Pseudomonas syringae pv. syringae (Pss), Pseudomonas syringae pv. lachrymans (Psl) and Pseudomonas tolaasii (P. tolaasii) were analyzed for genetic diversity. For molecular identification of the strains, three primer pairs were used. Molecular analysis was done with random amplified polymorphic DNA (RAPD) and insertion sequence (IS50) primers. IS50-PCR and RAPD-PCR analysis divided 51 strains into eight subclusters that were grouped in three clusters. Cluster I comprised all Pss with four subclusters from stone fruits, pome fruits and cereal hosts, cluster II contained Psl strains from cucumber with two subclusters and cluster III contained three strains of P. tolaasii with two subclusters from mushroom. These results indicated that a combination of IS50-PCR and RAPD-PCR fingerprinting can be used as a high resolution genomic fingerprinting method for differentiating species of Pseudomonas and pathovars of P. syringae strains.

引用

页码：487 / 491

页数：5

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