The volatile constituents of the oil extracted from the root, leaf and seed of Cleome quinquenervia DC were isolated through a hydro distillation method and then analyzed by GC and GC-MS.. Root, leaf and seed were found to contain 15, 52, and 42 components respectively. The major components of leaf oil were found to be beta-pinene (31 %), alpha-pinene (26.1 %), trans-pinocarvyl acetate (6.6 %). The root oil constituents were characterized by high amounts of alpha-eudesmol (29 %), beta-eudesmol (27.5 %) and gamma-eudesmol (13 %). The main constituents of the seed oil were shown to trans-pinocarvyl acetate (12.5 %), beta-eudesmol (10.8 %) and beta- pinene (10.8 %), alpha-eudesmol (9.6 %).