Determine the therapeutic and immunomodulatory effect of Aloe vera polysaccharides against colibacillosis in chickens

Poultry birds are exposed to different infectious diseases; among these infections, avian colibacillosis caused by E. coli is the most common in poultry birds. The irrational use of antibiotics in poultry results in resistance to disease-causing microorganisms, therefore now the world is moving towards plant treatment. Aloe vera has shown high antibacterial activity against E. coli in vivo and in vitro. Thus, the present study was designed to evaluate A. vera polysaccharides as a therapeutic and immunomodulatory agent against colibacillosis in chickens. E. coli was isolated and characterized by different outbreak cases of colibacillosis and identified by using the API-20E kit. The effects of A. vera polysaccharides as an immunomodulatory and therapeutic were evaluated in broiler chickens. Results exhibited a significant (p<0.05) lympho proliferative reaction to PHA-P chickens supplemented with A. vera polysaccharides in comparison to the control group. Carbon particle clearance test revealed significantly higher clearance index (K) in the control group, and the phagocytic index (alpha) showed a substantially higher response in all three polysaccharides supplemented groups in comparison to control. Total Igs, IgM, and IgG titers were also significantly higher in groups supplemented with A. vera polysaccharides. On challenge with pathogenic E. coli, more excellent protection was observed in all polysaccharides supplemented groups. The present study was suggested that A. vera extracted polysaccharides could be given as therapeutic and immunomodulatory agent(s) against colibacillosis in poultry.