Polyamine depletion inhibits NF-κB binding to DNA and interleukin-8 production in human chondrocytes stimulated by tumor necrosis factor-α

The activation of the NF-kappa B pathway by pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF alpha), can be an important contributor for the re-programming of chondrocyte gene expression, thereby making it a therapeutic target in articular diseases. To search for new approaches to limit cartilage damage, we investigated the requirement of polyamines for NF-kappa B activation by TNFa in human C-28/12 chondrocytes, using alpha-difluoromethylornithine (DFMO), a specific polyamine biosynthesis inhibitor. The NF-kappa B pathway was dissected by using pharmacological inhibitors or by expressing a transdominant I kappa B alpha super repressor. Treatment of C-28/12 chondrocytes with TNFa resulted in a rapid enhancement of nuclear localization and DNA binding activity of the p65 NF-kappa B subunit. TNF alpha also increased the level and extracellular release of interleukin-8 (IL-8), a CXC chemokine that can have a role in arthritis, in an NF-kappa B-dependent manner. Pre-treatment of chondrocytes with DFMO, while causing polyamine depletion, significantly reduced NF-kappa B DNA binding activity. Moreover, DFMO also decreased IL-8 production without affecting cellular viability. Restoration of polyamine levels by the co-addition of putrescine circumvented the inhibitory effects of DFMO. Our results show that the intracellular depletion of polyamines inhibits the response of chondrocytes to TNFa by interfering with the DNA binding activity of NF-kappa B. This suggests that a pharmacological and/or genetic approach to deplete the polyamine pool in chondrocytes may represent a useful way to reduce NF-kappa B activation by inflammatory cytokines in arthritis without provoking chondrocyte apoptosis.