Monitoring BCR-ABL transcript levels by real-time quantitative polymerase chain reaction: a linear regression equation to convert from BCR-ABL/B2M ratio to estimated BCR-ABL/ABL ratio

被引：7
|
作者：
Iacobucci, Ilaria [1 ]
Galletti, Livia [1 ]
Amabile, Marilina [1 ]
Soverini, Simona [1 ]
Baccarani, Michele [1 ]
Martinelli, Giovanni [1 ]
机构：
[1] Univ Bologna, S Orsola Malpighi Hosp, Dept Hematol Oncol L&A Seragnoli, Bologna, Italy
来源：
HAEMATOLOGICA | 2007年 / 92卷 / 03期
关键词：
BCR-ABL ratio; RT-PCR;
D O I：
10.3324/haematol.10632
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
In order to overcome the problem of different control genes for BCR-ABL normalization, we used a linear regression equation to compare our results previously obtained using B2M as the control gene with those calculated using the ABL gene and validated the slope as a factor to convert from B2M to ABL results.
引用
收藏
页码：429 / 430
页数：2
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