Radiotherapeutic bandage for the treatment of squamous cell carcinoma of the skin

Introduction: Squamous cell carcinoma (SCC) is the second most common form of skin cancer in the United States. The efficacy of a pharmaceutically elegant radiotherapeutic bandage, previously described by us for application against SCC of the skin, was tested for the first time in vivo using a subcutaneous SCC mouse model and a therapeutically relevant radiation dose. Methods: Female athymic nude mice were injected with human Colo-16 SCC cells subcutaneously and after eight days (average tumor volume: 35 +/- 8.6 mm(3)) received no treatment, or were exposed to non-radioactive or radioactive (92.5 +/- 18.5 MBq) bandages for approximately 1 h (n = 10 per group). After treatment, tumors were measured over fifteen days, tumor volume ratios (TVRs) compared and histopathology performed. Results: Fifteen days after treatment, the TVR of the radioactive bandage treatment group was 3.3 +/- 4.5, while TVRs of the non-radioactive bandage treatment and no treatment control groups were 33.2 +/- 14.7 and 26.9 +/- 12.6, respectively. At the time of necropsy, there was mild focal epidermal hyperplasia surrounding a small area of epidermal ulceration in the radioactive bandage group. No other examined tissue (i.e., muscle, liver, kidney, lung, spleen and heart) showed significant lesions. Conclusions: Our radiotherapeutic bandage exhibits promising efficacy against SCC of the skin in a mouse model. It can be individually tailored for easy application on tumor lesions of all shapes and sizes, and could complement or possibly replace surgery in the clinic. (C) 2016 The Authors. Published by Elsevier Inc.