The measurement of adhesion forces in the system "cell-cell" by using atomic force microscope

The objective was to develop a biosensor chip based on lymphocytes for studying the adhesion properties of the cells; to measure the adhesion forces between blood cells in normal and pathological conditions. In research standard cantilever CSG 11/tipless series (USA) was treated with 2% gelatin solution. After that, the lymphocyte was attached to it from the prepared leukocyte suspension placed on the glass. The preparation of the sensor was done in the power spectroscopy mode during the scan by single pressing on the cell. The patients' peripheral blood with acute lymphoblastic and acute myeloblastic leukemia was used for measuring adhesion forces among cells at the treatment and recurrence stage. The obtained data shows that the objective criteria of the beginning state of the recurrence of the lymphoproliferative processes is an increase in the forces of the intercellular interactions in the lymphocyte-granulocyte system, while the recurrence of myeloid lineage proliferation of haemopoiesis causes a decrease in adhesion forces in the same system. This result gives the possibility to predict and identify early micro-rheological changes at the beginning of decompensation of the remission state at the cellular level during the development of malignant proliferative processes in the blood system.