Specific localization of living cells in water/water microdroplets toward the spontaneous generation of 3D cell-assembly: Experiments with red blood cells and NAMRU mouse mammary gland epithelial cells

被引：0

作者：

Hoda, Y. ^{[1
]}

Fujimoto, T. ^{[1
]}

Yoshikawa, K. ^{[1
]}

Tsumoto, K. ^{[2
]}

机构：

[1] Doshisha Univ, Fac Life & Med Sci, Kyotanabe, Kyoto 6100321, Japan

[2] Mie Univ, Grad Sch Engn, Div Chem Mat, Tsu, Mie 5148507, Japan

来源：

2018 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS) | 2018年

关键词：

D O I：

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中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

We developed a new methodology to construct mini-organ, or organoid, in a self-organized manner from differently kinds of cells. It is getting clearer that aqueous environment around living eukaryotic cells plays an essential role to decide the fate-decision of stem cells. An aqueous solution in which multiple polymers such as polyethylene glycol (PEG) and dextran (DEX) are solubilized, undergoes phase separation, depending on its composition. In the present study, we have investigated the relationship between the polymer composition and the mode in which these cells are dispersed inside droplets. red blood cells (RBCs) and NAMRU mouse mammary epithelial cell (NMuMG cell) cells were located in the interior and at the periphery of the droplets at PEG/DEX = 5%:5%. In contrast, the cells exhibited opposite localizations at PEG/DEX = 10%:5%. We found innovative method that is automatic and low stress cell arrangement.

引用

页数：5