Mechanical and structural assessment of cortical and deep cytoskeleton reveals substrate-dependent alveolar macrophage remodeling

被引：0

作者：

Fereol, S. ^{[2
]}

Fodil, R. ^{[1
]}

Laurent, V. M. ^{[3
]}

Planus, E. ^{[4
]}

Louis, B. ^{[1
]}

Pelle, G. ^{[1
]}

Isabey, D. ^{[1
]}

机构：

[1] INSERM, CNRS, Fac Med,Inst Mondor Rech Biomed, Equipe Biomecan Cellulaire & Resp,UMR 841, F-94010 Creteil, France

[2] CNRS, UMR7101, Lab Neurobiol Signaux Intercellulaires Regenerat, Paris, France

[3] Univ Paris 05, Lab Neurophys Cellulaire, Paris, France

[4] IAB Site Sante, Equipe DySAD, Ctr Rech, INSERM,UJF,U823, Grenoble, France

来源：

BIO-MEDICAL MATERIALS AND ENGINEERING | 2008年 / 18卷

关键词：

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The sensitivity of alveolar macrophages to substrate properties has been described in a recent paper (Fereol et al., Cell Motil. Cytoskel. 63 (2006), 321-340). It is presently re-analyzed in terms of F-actin structure (assessed from 3D-reconstructions in fixed cells) and mechanical properties (assessed by Magnetic Twisting Cytometry experiments in living cells) of cortical and deep cytoskeleton structures for rigid plastic (Young Modulus: 3 MPa) or glass (70 MPa) substrates and a soft (similar to 0.1 kPa) confluent monolayer of alveolar epithelial cells. The cortical cytoskeleton component (lowest F-actin density) is represented by the rapid and softer viscoelastic compartment while the deep cytoskeleton component (intermediate F-actin density) is represented by the slow and stiffer compartment. Stiffness of both cortical and deep cytoskeleton is significantly decreased when soft confluent monolayer of alveolar epithelial cells replace the rigid plastic substrate while F-actin reconstructions reveal a consistent actin cytoskeleton remodeling observable on both cytoskeleton components.

引用

页码：S105 / S118

页数：14