Voltammetric Detection of a Specific DNA Sequence of Avian Influenza Virus H5N1 Using HS-ssDNA Probe Deposited onto Gold Electrode

被引：35
|
作者：
Malecka, Kamila [1 ]
Grabowska, Iwona [1 ]
Radecki, Jerzy [1 ]
Stachyra, Anna [2 ]
Gora-Sochacka, Anna [2 ]
Sirko, Agnieszka [2 ]
Radecka, Hanna [1 ]
机构：
[1] Polish Acad Sci, Inst Anim Reprod & Food Res, PL-10747 Olsztyn, Poland
[2] Polish Acad Sci, Inst Biochem & Biophys, PL-02106 Warsaw, Poland
来源：
ELECTROANALYSIS | 2012年 / 24卷 / 02期
关键词：
Electrochemical genosensor; HS-ssDNA probe; PCR products; Avian influenza virus; ION-CHANNEL SENSORS; LABEL-FREE DETECTION; ELECTROCHEMICAL DETECTION; CARBON NANOTUBES; NUCLEIC-ACIDS; HYBRIDIZATION; GRAPHENE; OLIGONUCLEOTIDE; IMMUNOSENSOR;
D O I：
10.1002/elan.201100566
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The working principle of a genosensor is based on the mechanism of ion-channel mimetic sensors. The analytical signals generated upon hybridization processes were recorded by a redox active marker [Fe(CN)6]3-/4- present in the sample solution using voltammetric techniques. The developed genosensor was suitable for determination of 20-mer complementary oligonucleotide sequence, and also of the PCR products containing the complementary 20-mer sequence in various positions, with detection limits in the 10 pM range. The noncomplementary 20-mer oligonucleotide sequence as well as the PCR product without complementary region generated very weak response. The good discrimination of the position of the complementary part in the PCR products was observed.
引用
收藏
页码：439 / 446
页数：8
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