Evolving Multiple Resistance to EPSPS, GS, ALS, PSI, PPO, and Synthetic Auxin Herbicides in Dominican Republic Parthenium hysterophorus Populations. A Physiological and Biochemical Study

被引：3

作者：

Palma-Bautista, Candelario ^{[1
]}

Hoyos, Veronica ^{[2
]}

Plaza, Guido ^{[3
]}

Vazquez-Garcia, Jose G. ^{[1
]}

Rosario, Jesus ^{[1
]}

Rojano-Delgado, Antonia M. ^{[1
]}

De Prado, Rafael ^{[1
]}

机构：

[1] Univ Cordoba, Dept Agr Chem & Edaphol, Cordoba 14071, Spain

[2] Magdalena Univ, Coll Engn, Magdalena 470004, Colombia

[3] Univ Nacl Colombia, Dept Agron, Bogota 111321, Colombia

来源：

AGRONOMY-BASEL | 2020年 / 10卷 / 04期

关键词：

Parthenium hysterophorus; Dominican Republic; multiple resistance; ACCASE-INHIBITING HERBICIDES; REDUCED TRANSLOCATION; GLYPHOSATE TOLERANCE; WEED MANAGEMENT; MECHANISMS; PARAQUAT; MODE; CONFIRMATION; GLUFOSINATE; METABOLISM;

D O I：

10.3390/agronomy10040554

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Two Parthenium hysterophorus populations resistant (R) and susceptible (S) harvested in banana crop from the Dominican Republic were studied. All S plants died when the herbicides were applied at field dose, except with paraquat. For the R population, the order of plant survival was as follows: glyphosate and paraquat > flazasulfuron > glufosinate > fomesafen > 2,4-D. The resistance factors obtained in the dose-response assays showed a high resistance to glyphosate, flazasulfuron, and fomesafen, medium resistance to glufosinate and 2,4-D, and a natural tolerance to paraquat (resistance factor (RF) = 1.0). The I-50 values obtained in the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), acetolactate synthase (ALS), and glutamine synthetase (GS) activity studies with glyphosate, flazasulfuron, and glufosinate, respectively, were greater in R than in S. The e ffect of fomesafen was measured by the Proto IX levels, obtaining five times more Proto IX in the S than in the R population. The resistance to 2,4-D in the R was determined by the lower accumulation of ethylene compared to the S population. The studies with C-14-paraquat conclude that the lower absorption and translocation in both the R and S populations would explain the natural tolerance of P. hysterophorus. This is the first case of multiple resistance to herbicides with different mechanisms of action confirmed in P. hysterophorus.

引用

页数：14