A colormetric assay for catechol-O-methyltransferase

被引：4
|
作者：
Bailey, K [1 ]
Cowling, R [1 ]
Tan, EW [1 ]
Webb, D [1 ]
机构：
[1] Univ Otago, Dept Chem, Dunedin, New Zealand
来源：
BIOORGANIC & MEDICINAL CHEMISTRY | 2004年 / 12卷 / 03期
关键词：
D O I：
10.1016/j.bmc.2003.10.038
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A series of catechol diazo dyes were synthesized and tested as substrates for the enzyme catechol-O-methyltransferase (COMT) with the aim of developing a sensitive HPLC assay method using visible wavelength light detection. A method was developed which allowed for the determination of the two regioisomeric methylated products of the COMT catalyzed reaction of 4-[(3,4-dihydroxyphenyl)azo]benzenesulfonate with S-adenosylmethionine (AdoMet). Separation of the assay components was achieved by reverse phase chromatography using an isocratic mobile phase. No pre-preparation of the assay samples was required. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码：595 / 601
页数：7
相关论文
共 50 条
  • [1] FLUORIMETRIC ASSAY FOR CATECHOL-O-METHYLTRANSFERASE
    OKADA, Y
    ZAITSU, K
    OHTSUBO, K
    NOHTA, H
    OHKURA, Y
    [J]. CHEMICAL & PHARMACEUTICAL BULLETIN, 1981, 29 (06) : 1670 - 1673
  • [2] CONTINUOUS SPECTROPHOTOMETRIC ASSAY FOR CATECHOL-O-METHYLTRANSFERASE
    COWARD, JK
    WU, FYH
    [J]. ANALYTICAL BIOCHEMISTRY, 1973, 55 (02) : 406 - 410
  • [3] RAPID SPECTROPHOTOMETRIC ASSAY FOR CATECHOL-O-METHYLTRANSFERASE
    BORCHARDT, RT
    [J]. ANALYTICAL BIOCHEMISTRY, 1974, 58 (02) : 382 - 389
  • [4] CATECHOL-O-METHYLTRANSFERASE
    FLOHE, L
    [J]. INTERNATIONAL PHARMACOPSYCHIATRY, 1974, 9 (01): : 52 - 60
  • [5] Rat liver catechol-O-methyltransferase kinetics and assay methodology
    Borges, N
    Vieira-Coelho, MA
    Parada, A
    Soares-da-Silva, P
    [J]. JOURNAL OF ENZYME INHIBITION, 1998, 13 (06): : 473 - 483
  • [6] Validation of assay of catechol-O-methyltransferase activity in human erythrocytes
    Tuomainen, P
    Reenila, I
    Mannisto, PT
    [J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1996, 14 (05) : 515 - 523
  • [7] CATECHOL-O-METHYLTRANSFERASE IN VITILIGO
    LEPOOLE, IC
    VANDENWIJNGAARD, RMJGJ
    SMIT, NPM
    OOSTING, J
    WESTERHOF, W
    PAVEL, S
    [J]. ARCHIVES OF DERMATOLOGICAL RESEARCH, 1994, 286 (02) : 81 - 86
  • [8] Inhibitors of Catechol-O-Methyltransferase
    Barrow, James C.
    [J]. CNS & NEUROLOGICAL DISORDERS-DRUG TARGETS, 2012, 11 (03) : 324 - 332
  • [9] CATECHOL-O-METHYLTRANSFERASE IN VITILIGO
    LEPOOLE, IC
    VANDENWIJNGAARD, RMJGJ
    SMITH, NPM
    OOSTING, H
    WESTERHOF, W
    PAVEL, S
    [J]. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1993, 100 (04) : 456 - 456
  • [10] CATECHOL-O-METHYLTRANSFERASE .I. AN ENZYMATIC ASSAY FOR CARDIAC NOREPINEPHRINE
    NIKODIJEVIC, B
    DALY, J
    CREVELING, CR
    [J]. BIOCHEMICAL PHARMACOLOGY, 1969, 18 (07) : 1577 - +
12345