Cloning and Sequence Analysis of Rabbit Interleukin-10 (IL-10) Gene Promoter

被引：0

作者：

Mao Liu-Liu ^{[1
,3
]}

Pan Yu-Lai ^{[2
]}

Wan Xiao-Ying ^{[1
,3
]}

Wang Xiao-Ming ^{[1
,3
]}

Feng Kai ^{[1
,3
]}

Zhang Lei ^{[1
,3
]}

He Meng-Jie ^{[1
,3
]}

Wu Xin-Sheng ^{[1
,3
]}

机构：

[1] Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Jiangsu, Peoples R China

[2] Jinling Rabbits Farm, Nanjing 211103, Peoples R China

[3] Yangzhou Univ, Jiangsu Key Lab Anim Genet & Breeding & Mol Desig, Yangzhou 225009, Jiangsu, Peoples R China

来源：

JOURNAL OF ANIMAL AND VETERINARY ADVANCES | 2012年 / 11卷 / 03期

关键词：

IL-10; promoter; transcription factor; bioinformatics; gene; China; TRANSCRIPTIONAL REGULATION; RECEPTOR;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

To clone the IL-10 promoter of rabbit and analyse its sequence, this study amplified the 5 flanking region of rabbit IL-10, compared the genomes and used bioinformatics software to make a forecast. Then, the researchers comfimied partial promoter sequences and analysed sonic transcription factors of the IL-10 gene promoter. The study showed that rabbit and human IL-10 gene promoter had some similarities which all cotained GAGA, CAAT and TATA. The transcriptional start site started from -80 bp of translation initiation codon (ATG) upstream region. The promoter also had some transcription factors such as AP-1, Myogenin, c-Ets-1, C/EBP alpha and so on, Results of this study predicted partial sequences, transcriptional start site and some transcriptional regulating factors binding sites of IL-10 gene promoter which would lay the foundation of lucubrating the expression and regulation of rabbit IL-10 gene.

引用

页码：323 / 326

页数：4

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