Development of screening methods for transketolase activity and substrate scope

被引：8
|
作者：
Ranoux, Adeline [1 ]
Arends, Isabel W. C. E. [1 ]
Hanefeld, Ulf [1 ]
机构：
[1] Delft Univ Technol, Afdeling Biotechnol, NL-2628 BL Delft, Netherlands
来源：
TETRAHEDRON LETTERS | 2012年 / 53卷 / 07期
关键词：
Transketolase; Screening methods; Substrate scope; Selectivity; Mutant libraries; DIRECTED EVOLUTION; TRIMETHYLSILYL DERIVATIVES; SUGARS; STEREOSELECTIVITY; CHROMATOGRAPHY; ACIDS; ASSAY;
D O I：
10.1016/j.tetlet.2011.12.010
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A new GC-based method is developed for the screening of activity and stereoselectivity of a wide range of polyols as substrates of transketolase (TK). Compared to previous screening methods, it shows higher reproducibility, sensitivity and range of detection. In combination with HPLC screening, it can be used efficiently to test mutant libraries obtained by directed evolution methods. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码：790 / 793
页数：4
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