Evaluation of acetylcholinesterase and butyrylcholinesterase inhibitory activity of Huperzine-A; in silico and in vitro studies

被引：0
|
作者：
Upadhyay, Shagun Dubey [1 ]
Ahmad, Yusra [2 ]
Kohli, Seema [1 ]
Sharma, R. K. [3 ]
机构：
[1] Govt Kalaniketan Polytech Coll, Dept Pharm, Jabalpur 482001, Madhya Pradesh, India
[2] Uttarakhand Tech Univ, Fac Pharm, Dehra Dun 248007, Uttarakhand, India
[3] Nanaji Deshmukh Univ Vet Sci, Coll Vet Sci & Anim Husb, Dept Pharmacol & Toxicol, Jabalpur 482001, Madhya Pradesh, India
来源：
INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS | 2019年 / 56卷 / 03期
关键词：
Acetylcholinesterase; Butyrylcholinesterase; Cognition; Huperzine-A; GENETIC-VARIANTS; DOCKING; RATS;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The present study is focused on exploring the Acetylcholinesterase and Butyrylcholinesterase inhibitory activity of Huperzine-A in silico and in vitro. In this study, Huperzine-A-A was docked with Acetylcholinesterase and Butyrylcholinesterase. Docking studies revealed the excellent interaction of Huperzine-A-A with these targets. The result of present study provides insight for the in vitro studies. The in vitro studies the enzyme kinetics of Huperzine-A-A via Lineweaver brooks plot revealed the kinetics and non-competitive inhibitory nature of the later. Further studies on Huperzine-A-A are necessary to develop and establish its role on brain cholinergic system and cognitive deficits which may serve a stepping stone in CNS medication.
引用
收藏
页码：224 / 229
页数：6
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