ANALYTICAL PROCEDURE FOR THE DETERMINATION OF TYLOSIN A IN FEEDINGSTUFF BY LIQUID CHROMATOGRAPHY-ULTRAVIOLET DETECTION

A reliable and selective liquid chromatography ultraviolet detection method for determination of tylosin has been developed. The extraction of analyte from feedingstuffs was performed with solution of citric buffer and methanol. The extracts were cleaned up by solid phase extraction procedure using a octadecyl cartridge. Samples were brought up to dryness and dissolved in phosphate buffer. The analysis was carried out on C18 analytical column with UV detection at lambda = 282 nm. The analytical procedure has been successfully adopted and validated for quantitative determination of tylosin A in feedingstuff samples. The validation included determination of specificity, linearity, repeatability, and within-laboratory reproducibility. Mean recovery for spiked samples was 84.7% within the working range of 5-1,000 mg/kg. The inter-day relative standard deviation was below 6.6%. The results of validation procedure proved that presented method is efficient, precise, and useful for routine analysis for screening of quality, homogeneity, and stability of medicated feedingstuffs.