Lysobacter ciconiae sp. nov., and Lysobacter avium sp. nov., isolated from the faeces of an Oriental stork

Two Gram-stain-negative, mesophilic, strictly aerobic, nonspore forming, and yellow-pigmented strains with rod-shaped cells, designated H21R20(T) and H23M41(T), were isolated from the faeces of an Oriental stork (Ciconia boyciana). Based on 16S rRNA gene sequences, both strains showed the highest similarity (98.3-98.4%) to the type strain of Lysobacter concretionis. Phylogenetic analysis based on the 16S rRNA genes and 92 bacterial core genes showed that strains H21R20(T) and H23M41(T) were robustly clustered with L. concretionis Ko07(T). Whole genome sequencing revealed that the genomes of both strains were approximately 2.9 Mb in size. The DNA G + C contents of the H21R20(T) and H23M41(T) strains were 67.3 and 66.6%, respectively. The two strains showed 80.1-81.7% average nucleotide identity with L. concretionis Ko07(T). Strain H21R20(T) grew optimally at 30 degrees C and pH 8.0 and in the presence of 0.5-3% (wt/vol) NaCl, while strain H23M41(T) grew optimally at 30 degrees C and pH 7.0-8.0 and in the presence of 0-3% (wt/vol) NaCl. Both strains possessed iso-C-15:0, iso-C-16:0 and summed feature 9 (iso-C-17:1 omega 9c and/or C(16:0)10-methyl) as the major cellular fatty acids, ubiquinone Q-8 as a predominant quinone, and diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine as the major polar lipids. A multifaceted investigation demonstrated that strains H21R20(T) and H23M41(T) represent novel species of the genus Lysobacter, for which we propose the names Lysobacter ciconiae sp. nov. and Lysobacter avium sp. nov. for strains H21R20(T) (= KCTC 82316(T) = JCM 34832(T)) and H23M41(T) (= KCTC 62676(T) = JCM 33223(T)), respectively.