Antimicrobial Susceptibility and PFGE Genotyping of Haemophilus parasuis Isolates from Pigs in South China (2008-2010)

被引:13
|
作者
Xu, Chenggang [1 ]
Zhang, Jianmin [1 ]
Zhao, Zhanqin [2 ]
Guo, Lili [1 ]
Zhang, Bin [1 ]
Feng, Saixiang [1 ]
Zhang, Lingyun [1 ]
Liao, Ming [1 ]
机构
[1] S China Agr Univ, Key Lab Anim Dis Control & Prevent, Minist Agr,Coll Vet Med, Key Lab Zoonoses Prevent & Control Guangdong, Guangzhou, Guangdong, Peoples R China
[2] Henan Univ Sci & Technol, Luoyang, Henan, Peoples R China
来源
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2011年 / 73卷 / 08期
基金
中国国家自然科学基金;
关键词
antimicrobial susceptibility; Haemophilus parasuis; PFGE; swine; RESISTANCE; EPIDEMIOLOGY; PASTEURELLA;
D O I
10.1292/jvms.10-0515
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
H. parasuis isolates (n=112) from pigs were tested for antimicrobial susceptibility against 23 antimicrobial agents by the disk diffusion method. All isolates were sensitive to Florfenico and most strains were sensitive to Cefotaxime (103/112; 92%), Ceftazidime (99/112; 88.4%), Chloramphenicol (90/112; 80.4%) and Gentamicin (85/112; 75.9%). High resistance levels to Nalidixic acid (84.8%), TMP (67.9%) and Trimethoprim+Sulfamethoxazole (58%) were observed. Genomic DNA extracted from 52 isolates resistant to at least seven antimicrobial agents was analyzed by PFGE and 46 distinct PFGE patterns identified. Diverse variation was observed between the drug-resistant H. parasuis isolates examined, suggesting that resistance traits were acquired independently by the respective isolates.
引用
收藏
页码:1061 / 1065
页数:5
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