Performance of an amperometric biosensor for the determination of hemoglobin

Hemoglobin is a very important physiological index in human blood, especially for the evaluation of the physical performance of athletes. This paper reports an amperometric biosensor for the determination of blood hemoglobin, using potassium ferricyanide as a mediator, based on thin-film technology in a two-electrode system. The biosensor can be used for the determination of hemoglobin at concentrations in a linear range of 10-3000 mu M, and the correlation coefficient is 0.996, the relative standard deviation is 2.3% for six determinations for 2000 mu M hemoglobin concentration. And the linear range of hemoglobin is studied using methylene blue as a mediator.