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Protective effects of MLIF analogs on cerebral ischemia-reperfusion injury in rats
被引:15
|作者:
Yao, Jia
[2
,3
,4
]
Xu, Ying
[5
]
Ji, Fang
[1
]
Wang, Chao
[1
]
Zhang, Yun
[1
]
Ni, Jingman
[1
]
Wang, Rui
[2
,3
,4
]
机构:
[1] Lanzhou Univ, Sch Pharm, Lanzhou 730000, Peoples R China
[2] Lanzhou Univ, Key Lab Preclin Study New Drugs Gansu Prov, Sch Basic Med Sci, Lanzhou 730000, Peoples R China
[3] Lanzhou Univ, Inst Biochem & Mol Biol, Sch Life Sci, Lanzhou 730000, Peoples R China
[4] Lanzhou Univ, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China
[5] Gansu Tumor Hosp, Gansu Prov Med Sci Inst, Lanzhou 730050, Gansu, Peoples R China
来源:
基金:
中国国家自然科学基金;
关键词:
Monocyte locomotion inhibitory factor;
Neuroprotective;
Anti-inflammatory;
Inflammatory cytokine;
N-terminal modified;
INHIBITORY FACTOR MLIF;
HUMAN MONOCYTE LOCOMOTION;
ENTAMOEBA-HISTOLYTICA;
ARTERY OCCLUSION;
FOCAL ISCHEMIA;
BRAIN-DAMAGE;
TNF-ALPHA;
EXPRESSION;
STROKE;
INTERLEUKIN-1-BETA;
D O I:
10.1016/j.peptides.2011.03.005
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
The monocyte locomotion inhibitory factor (MLIF) is an anti-inflammatory oligopeptide produced by Entamoeba histolytica. Among its different effects, it inhibits locomotion of human monocytes, hence its original name. The carboxyl-terminal end group Cys-Asn-Ser is the pharmacophore of anti-inflammatory peptide Met-Gln-Cys-Asn-Ser. In this study, the N-terminal of Cys-Asn-Ser was modified. With the aim to enhance the antioxidant ability and penetrability of Cys-Asn-Ser, we designed and synthesized two tetrapeptides Tyr-Cys-Asn-Ser and His-Cys-Asn-Ser. The neuroprotective effects of Tyr-Cys-Asn-Ser and His-Cys-Asn-Ser on focal ischemia reperfusion were investigated, and their pharmacological activities compared with Cys-Asn-Ser were studied. In order to study the mechanism of neuroprotective effect of these peptides, the level of oxidative stress markers malondialdehyde (MDA) and superoxide dismutase (SOD) and pro-inflammatory factors interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), and myeloperoxidase (MPO) were detected in brain tissue homogenate. (C) 2011 Elsevier Inc. All rights reserved.
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页码:1047 / 1054
页数:8
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