Hepatoprotective effects of Pandanus amaryllifolius against carbon tetrachloride (CCl4) induced toxicity: A biochemical and histopathological study

The purpose of this study was to determine the phytochemical constituents of Pandanus amaryllifolius as well as to evaluate its ability to protect against acute hepatic damage caused by carbon tetrachloride (CCl4) in rats. In animals pre-treated with P.amaryllifolius and intoxicated with CCl4, biochemical parameters such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutathione (GSH), catalase (CAT) and malondialdehyde (MDA) were used to assess hepatic damages. Histopathological assessment was also done to evaluate the CCl4 mediated hepatic injury in rats. P.amaryllifolius antioxidant activity was measured using free radical scavenging DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The stable DPPH level was lowered by P. amaryllifolius extract. The value of the half-maximum inhibitory level (IC50) was 46.8 mu g/ml. Phytochemical screening of P. amaryllifolius extract showed the presence of tannins, alkaloids, saponins, terpenoids and flavonoids except pholabatannins and cardiac glycosides. The total phenolic content (TPC) was 35.99 +/- 0.04 mg GAE/g and total flavonoid content (TFC) was 59. 96 +/- 0.013 mg CAE/g of extract. P.amaryllifolius pre-treated groups displayed significantly increased catalase antioxidant enzyme activity relative to the CCl4 treated group (57-82%, P < 0.05). P.amaryllifolius was found to moderately reduce serum ALT and AST levels (4-34%, P < 0.05). The formation of MDA due to lipid peroxidation was greatly reduced (29-70%, P < 0.05) when compared to the CCl4 treated group, while GSH was raised in a dosage dependent way (94-100%, P < 0.05). Reduced histological changes in the liver were clear evidence of P. amaryllifolius protective effect. According to the research findings, the antioxidant and free radical scavenging properties of P. amaryllifolius may be responsible for its hepatoprotective benefits. (C) 2021 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.