Thidiazuron induces high frequency shoot bud formation from Begonia petiole transverse thin cell layer culture

被引：0
|
作者：
Nhut, DT
Hai, NT
Huyen, PX
Huong, DTQ
Hang, NTT
da Silva, JAT
机构：
[1] Dalat Inst Biol, Dept Plant Biotechnol, Da Lat, Lam Dong, Vietnam
[2] Kagawa Univ, Fac Agr, Kagawa 7610795, Japan
来源：
PROPAGATION OF ORNAMENTAL PLANTS | 2005年 / 5卷 / 03期
关键词：
Begonia; thidiazuron; shoot bud formation; tTCL;
D O I：
暂无
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
The effect of thidiazuron (TDZ) on shoot bud formation of Begonia spp. using transverse thin cell layer (tTCL) technology is presented in this paper. tTCLs were cut from Begonia petioles about 0.8-1.0 mm thick and cultured on half-strength MS medium supplemented with various concentrations of BA, TDZ, and different combinations of BA or TDZ and NAA. The highest shoot bud formation was obtained on medium with 0.2 mg l(-1) TDZ and 0.2 mg l(-1) NAA, giving rise to 100% shoot formation after 8 weeks. TDZ at low concentrations (0.1-0.2 mg l(-1)) was effective for caulogenesis from Begonia tTCLs.
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页码：151 / 157
页数：7
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